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TOOLS AND STRATEGIES FOR BIOMARKER TRANSLATION

This site brings together the latest articles, resources, and peer-reviewed publications with a focus on reliable and precise protein quantitation. Power the translation of your biomarker discovery program with Waters targeted workflow and overcome the limitations and risks of immunoassay techniques.

Three Col Intro

Immunoassay
Challenges

Immunoassays have supported biomarker discovery for many years. But due to the inherent variability of antibodies, those discoveries aren’t always valid and reproducible. This so-called crisis of reproducibility is driving a powerful movement to improve the validity of published science.

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LC-MS Solutions

LC-MS workflows compare favorably with antibody-based techniques, such as ELISAs or protein arrays. The methods are more reproducible and can be developed more rapidly during the early and late validation/verification stages of protein or metabolite biomarkers.

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What's New

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Register now for periodic news updates on biomarker discovery and validation/verification peer-reviewed articles, technologies and applications.

REMOVE THE VARIABLES FROM YOUR PROTEIN & PEPTIDE QUANTIFICATION WORKFLOW

Translational biomarker discovery demands consistency and reproducibility. Yet traditional workflows rely heavily on antibody-based methods, which can introduce variables at any development phase. Antibodies often suffer from non-sensitivity, non-specificity and lot-to-lot variability, making it difficult – if not impossible – to verify biomarker results.

Immunoassays: Costs and Risks

  • Antibodies require long development times and add significant cost to the study (del Campo, Front. Neurol. 2015)
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  • Cost and development times of antibodies limit the number of biomarkers moving from discovery into verification (Makawita, Clinical Chemistry 2009)
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  • Antibodies are prone to cross-reactivity, increasing risks of erroneous data (Forseca, Nature Cell Biology, 2014)
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  • Lot-to-lot variability of reagents introduces irreproducibility and increases the risk of false negatives/positives (Baker, Nature, 2015)
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Explore the Latest Publications

STREAMLINE YOUR BIOMARKER WORKFLOW WITH MORE PRECISE AND RELIABLE TOOLS

Moving from immunoassay to LC-MS-based methods can streamline biomarker workflows and deliver accurate, reproducible results. Scientists can evaluate multiple biomarkers in a single assay and rapidly develop new methods during early and late validation/verification of protein or metabolite biomarkers.

The Benefits of LC-MS

  • Shortened assay development timelines with no additional reagent cost
  • Simultaneous quantification of multiple biomarkers in limited sample volumes empowers a deeper understanding of biological activity
  • Accurate and precise quantification
  • Reduced analytical variability by maintaining a consistent platform across discovery, validation and verification stages
  • Flexible analytical platform for a variety of target analytes

Explore the Latest Advances

  • Reproducible Results

    Simplified protocols increase workflow efficiencies and eliminate risks of antibody derived variability

  • Save Money and Time

    Removes the need to develop antibodies, reducing time-to-results and the cost of studies

  • Flexibility

    Measures multiple biomarkers in a single analysis with a high degree of accuracy and specificity

Quote: Journal of Immunological Methods

“Antibodies are the workhorse of biological experiments—and they are littering the field with false findings.”

Nature, 2015

Compare LC-MS to Immunoassay Methods

The ability to simultaneously measure a large number of biomarkers – with a high degree of specificity and precision – can relieve one of the greatest pain points in modern biology.

Evaluate the Methods

Discover how your peers are using LC-MS-based validation methodologies to make a dramatic difference in their translational workflow:

Quote: Nature

“Conventional approaches of studying immunological responses to exposure or infection such as Enzyme-Linked Immunosorbent Assays (ELISAs) and other monoplex antibody-based immunoassays can be very costly, laborious, and consume large quantities of sample. A major limitation of these approaches is that they can only be used to measure one analyte at a time."

Journal of Immunological Methods, 2015

Case Studies

Meeting the demands of translational biomarker discovery laboratories is an awesome responsibility. Waters provides advanced tools for protein discovery, characterization, quantification and validation. Hear why thought leaders turn to Waters for the best-in-class technologies that will help them meet their toughest challenges.

LC-MS for Food Science

Dr. Jessica Prenni and her team at Colorado State University's (CSU) Proteomics and Metabolomics Facility discuss how the next discovery in food science may come from the field of metabolomics and the significant role the ionKey/MS system is playing.

LC-MS for Tumor Associated Antigens (TAA)

Duke University’s Proteomics and Metabolomics Core Facility details how Waters’ ionKey/MS technology quantifies tumor associated antigens, and the impact this could have on healthcare.

Translational Research With ionKey/MS

ionKey/MS, coupled with ACQUITY UPLC
M-Class System offers superior performance and 5-40x improvement in sensitivity for high-throughput quantification of peptides, proteins, and small molecules. Simple plug-n-play architecture makes ionKey/MS an ideal quantitative solution for biomarker verification and validation.

With its ease-of-use, reliable micro-fluidics, reduced sample consumption, and enhanced sensitivity, ionKey/MS technology provides the streamlined solution you need to answer complex biological questions.

The Science of ionKey/MS

Taking Advantage of Significant Reductions in Ion Suppression Using ionKey/MS Compared to Standard-Flow LC-MS (Johnson, Waters White Paper)

Enhancing Mass Spectrometry Sensitivity By Reducing Chromatographic Flow Rates with ionKey/MS (Murphy, Waters White Paper)


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FOLLOW THE FIELD:

Register now for periodic news updates on biomarker discovery and validation/verification peer-reviewed articles, technologies and applications.